| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.041499 |
| Chromosome: | chromosome 10 |
| Location: | 889779 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424100 | IPY1 | (1 of 1) PTHR10286//PTHR10286:SF3 - INORGANIC PYROPHOSPHATASE // INORGANIC PYROPHOSPHATASE; Inorganic pyrophosphatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGTGATGCAGCTTGAGGTCTAATGCGCC |
| Internal bar code: | AAGCGCCCAGGTTACAAATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 956 |
| LEAP-Seq percent confirming: | 99.2377 |
| LEAP-Seq n confirming: | 5728 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTATGCGGAAGCAGCAGT |
| Suggested primer 2: | TATGTCAGAGCTGTGGGCTG |