Insertion junction: LMJ.RY0402.041510_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.041510
Chromosome:chromosome 1
Location:1944444
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g010350 LIPB1,LPL1 (1 of 1) PTHR10993//PTHR10993:SF7 - OCTANOYLTRANSFERASE // LIPOYLTRANSFERASE 2, MITOCHONDRIAL-RELATED; Lipoate protein ligase B 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTACGACCAACGGACCTGCACCCTGTGCA
Internal bar code:CAAACTTGGTGCAGGAAGAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:476
LEAP-Seq percent confirming:99.7613
LEAP-Seq n confirming:836
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTGTGCGTATCCAACCTT
Suggested primer 2:ACTTAACCCTCCCACGCTTT