| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.041523 |
| Chromosome: | chromosome 2 |
| Location: | 6087242 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113050 | (1 of 2) PTHR23344:SF28 - GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGTGGGTGCAGGGTCAAGATGTTTTGC |
| Internal bar code: | AAGTGGTTGGTTTATTTCATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 434 |
| LEAP-Seq percent confirming: | 98.0952 |
| LEAP-Seq n confirming: | 103 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCACACTACCCTGCCAGT |
| Suggested primer 2: | TTGTCGCTTTCATCACTTGC |