| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.041528 |
| Chromosome: | chromosome 2 |
| Location: | 7315303 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g145300 | (1 of 1) K15544 - RNA polymerase II subunit A C-terminal domain phosphatase SSU72 (SSU72) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTGGAACCTCTCCGGCGCGGTCTTGAC |
| Internal bar code: | CGCGATACGATTTGGGGTCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 244 |
| LEAP-Seq percent confirming: | 99.6169 |
| LEAP-Seq n confirming: | 1300 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATAGCCACAGATACCGCC |
| Suggested primer 2: | GCTGCGGGTTTTGTTATGTT |