| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.041543 |
| Chromosome: | chromosome 12 |
| Location: | 5609371 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532050 | (1 of 1) PF00069//PF04564//PF07714//PF12796 - Protein kinase domain (Pkinase) // U-box domain (U-box) // Protein tyrosine kinase (Pkinase_Tyr) // Ankyrin repeats (3 copies) (Ank_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCACGCAGCGAAGCGCCAGGTCCGCGAA |
| Internal bar code: | TTTCACATTTGCGCTCAAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 768 |
| LEAP-Seq percent confirming: | 95.9908 |
| LEAP-Seq n confirming: | 838 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGAGCCAGCAATACGATG |
| Suggested primer 2: | TCGAGTACATGTGAGCTGGC |