| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.041546 |
| Chromosome: | chromosome 9 |
| Location: | 2767156 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389150 | FAP93 | Flagellar Associated Protein 93 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTGAGCGTCGCCGCCGCCAATAAGTTC |
| Internal bar code: | AAGGATCTCCTTGCCCCCCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 875 |
| LEAP-Seq percent confirming: | 97.4516 |
| LEAP-Seq n confirming: | 6348 |
| LEAP-Seq n nonconfirming: | 166 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCAAGTGTAGGAGGGAG |
| Suggested primer 2: | ACAGCTGGTACATGCGTGAG |