| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.041568 |
| Chromosome: | chromosome 4 |
| Location: | 3679131 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g228925 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTAATGAGGTCGCAAACGCGGTAGAGCA |
| Internal bar code: | TTCCGATCTATGCTGAGCGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 714 |
| LEAP-Seq percent confirming: | 85.8618 |
| LEAP-Seq n confirming: | 5156 |
| LEAP-Seq n nonconfirming: | 849 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACAGGCGATGTAAGACAA |
| Suggested primer 2: | AGGGGTAGCACCACACAGAC |