| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.041603 |
| Chromosome: | chromosome 5 |
| Location: | 2469844 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g235550 | OPR125 | OctotricoPeptide Repeat protein 125; (1 of 3) IPR016030 - Adenosylcobalamin biosynthesis, ATP:cob(I)alamin adenosyltransferase-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTCAGGTCCCGGGCGGCGTCGGCGCTG |
| Internal bar code: | GAGACCGCAGGGGGGAGCAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 357 |
| LEAP-Seq percent confirming: | 98.3206 |
| LEAP-Seq n confirming: | 644 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAAGCTCTGCAGCTCCTC |
| Suggested primer 2: | AGATGGTAGTGGATGCCAGG |