| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.041607 |
| Chromosome: | chromosome 3 |
| Location: | 5056039 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g180900 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGTGCGCTTGGCTGCGTGCGCTACAGCT |
| Internal bar code: | CTGGTCGTGGGTTACCACCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 52 |
| LEAP-Seq percent confirming: | 75.8261 |
| LEAP-Seq n confirming: | 436 |
| LEAP-Seq n nonconfirming: | 139 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGAAGTTGTGATTCCGT |
| Suggested primer 2: | CCAGAAGGCAGGACATCAGT |