Insertion junction: LMJ.RY0402.041627_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.041627
Chromosome:chromosome 4
Location:2860462
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g224400 (1 of 1) 3.6.3.25//3.6.3.44 - Sulfate-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAAACGCCCACGCTGTCGGCGGCGAAAGG
Internal bar code:AGGGTGAAGGGATCGAAAGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:309
LEAP-Seq percent confirming:82.4026
LEAP-Seq n confirming:1269
LEAP-Seq n nonconfirming:271
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGATGCAAATGCAAGATG
Suggested primer 2:CAGACACCCGAACTGACAGA