| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.041632 |
| Chromosome: | chromosome 1 |
| Location: | 6648837 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g047650 | (1 of 1) K10268 - F-box and leucine-rich repeat protein 2/20 (FBXL2_20) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGTCCTGCAGCGTCAGGCCGCGGAGGT |
| Internal bar code: | CCTCGTGTGGCCCAGAGCCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 228 |
| LEAP-Seq percent confirming: | 97.5904 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCGAACAGTAAGGGGAT |
| Suggested primer 2: | GCGTGCTGACTTTGTGTGTT |