| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.041684 |
| Chromosome: | chromosome 17 |
| Location: | 3301534 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723200 | FAP372 | Flagellar Associated EF Hand Protein 372 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACACGATGTTCCGGAACCTCAGTGTGC |
| Internal bar code: | GTGTACGGTATCAGGCAGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 612 |
| LEAP-Seq percent confirming: | 93.2979 |
| LEAP-Seq n confirming: | 877 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATCTTCACCGCTCATGCT |
| Suggested primer 2: | ATGCCTTTCCGTGTTACGTC |