| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.041685 |
| Chromosome: | chromosome 6 |
| Location: | 957723 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g255600 | EZY24,EZY1,EZY1A | Copy A in the EZY1 tandem gene cluster | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCATCATACAGTATTCAATCCACTTTGT |
| Internal bar code: | AGGATGTTCCTTGGTACGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 96.5812 |
| LEAP-Seq n confirming: | 1808 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTATGTGATGTGACAGCG |
| Suggested primer 2: | TGGATGGGGTAACCTGTGTT |