Insertion junction: LMJ.RY0402.041715_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.041715
Chromosome:scaffold_21
Location:125898
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
None

Insertion site details

Flanking sequence (orientation from cassette outwards):TGCGCGCACAGCGCCGCCTGCCAGTTCCGC
Internal bar code:GGATACCGCGGACAGTGTAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1413
LEAP-Seq percent confirming:98.6644
LEAP-Seq n confirming:1182
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGACTTGCACCCATGTTT
Suggested primer 2:TTGTTGCCGTCATCAAACAT