Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.041733 |
Chromosome: | chromosome_16 |
Location: | 6797761 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g674515 | IDA7 | Flagellar inner dynein arm I1 intermediate chain IC140 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGTTCTCCTCGCCCTCGGGCTTGACGAAAT |
Internal bar code: | TAACCGGTGAGGATCGGGGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 937 |
LEAP-Seq percent confirming: | 99.1078 |
LEAP-Seq n confirming: | 5110 |
LEAP-Seq n nonconfirming: | 46 |
LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGCCCATCAGCTTCTTCTC |
Suggested primer 2: | AGGTTTTGTTCTGGGACGTG |