Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.041737 |
Chromosome: | chromosome_5 |
Location: | 2807372 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g237283 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATTGTCCGCGCATGGCAAGCGCTTATGGCC |
Internal bar code: | CTTCGTGATCATTTCGGGCCCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 537 |
LEAP-Seq percent confirming: | 80.5405 |
LEAP-Seq n confirming: | 298 |
LEAP-Seq n nonconfirming: | 72 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACGTTGAGTGCATTGGGAA |
Suggested primer 2: | AGGTTTAATGACTGGTGCGG |