| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.041742 |
| Chromosome: | chromosome 7 |
| Location: | 2431317 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328900 | CDPK3,CDPK9 | (1 of 6) PTHR24349//PTHR24349:SF23 - SERINE/THREONINE-PROTEIN KINASE // MAP KINASE-ACTIVATED PROTEIN KINASE 5; Calcium/calmodulin-dependent protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACGCCCGGCTACCGGGCGCCTCAGCTCG |
| Internal bar code: | ATACCCACATGTTGGTGGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 839 |
| LEAP-Seq percent confirming: | 99.7497 |
| LEAP-Seq n confirming: | 13152 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCTAGAGCAGGAGCTGA |
| Suggested primer 2: | GCGTTGAAGGCATACCAAAT |