| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.041753 |
| Chromosome: | chromosome_1 |
| Location: | 4663824 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g032150 | FAP187 | Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTCGGTGTTTTCGACTCTCATAGATGCGCT |
| Internal bar code: | ACCGTTACGGTCACGGAACTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 361 |
| LEAP-Seq percent confirming: | 73.0989 |
| LEAP-Seq n confirming: | 769 |
| LEAP-Seq n nonconfirming: | 283 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCAATAGAAAGACACGGC |
| Suggested primer 2: | GCAATCTATGCCACACCCTT |