| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.041758 |
| Chromosome: | chromosome 3 |
| Location: | 3127209 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165000 | EFG10,LPA1 | (1 of 1) K03596 - GTP-binding protein LepA (lepA); Translation elongation factor EFG/EF2, LepA-related | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTCTCCTGAGCGCTTCAGTACTCCTTCG |
| Internal bar code: | TTATAAGTTGGATCGACAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 385 |
| LEAP-Seq percent confirming: | 99.3924 |
| LEAP-Seq n confirming: | 1145 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGACCTGGAGGTAATCCC |
| Suggested primer 2: | AAGCGAAATTGTAGCGAGGA |