| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.041774 |
| Chromosome: | chromosome 7 |
| Location: | 5924217 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g354150 | MFT22,ARSJ1 | Major facilitator superfamily transporter; (1 of 82) IPR020846 - Major facilitator superfamily domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCCTGGTACCTCCCCCATCCATGCACAT |
| Internal bar code: | GCTAAATGATTAGGATTTGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 336 |
| LEAP-Seq percent confirming: | 99.6512 |
| LEAP-Seq n confirming: | 11998 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTTCGATCTCAAACACG |
| Suggested primer 2: | AACTTACCACCCAGCCCTCT |