| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.041777 |
| Chromosome: | chromosome 11 |
| Location: | 2777647 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATATGCTGCAGCACCCGCCACAATGGCC |
| Internal bar code: | ACTCTACGTGCATCATTTTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 95.0775 |
| LEAP-Seq n confirming: | 7050 |
| LEAP-Seq n nonconfirming: | 365 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGGTCCCTGTGATGGTTG |
| Suggested primer 2: | TTACTTGTGGCAAACCCCTC |