| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.041804 |
| Chromosome: | chromosome 12 |
| Location: | 4685251 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523300 | RPN1A,OST1A,GTR22 | Oligosaccharyltransferase subunit 1A, Ribophorin-1A; (1 of 1) K12666 - oligosaccharyltransferase complex subunit alpha (ribophorin I) (OST1, RPN1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCGCACCTGGCCCGCGACACGGGCGGA |
| Internal bar code: | GCTGGGGCTACCGGTAGTGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 708 |
| LEAP-Seq percent confirming: | 99.5768 |
| LEAP-Seq n confirming: | 7764 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGACCAGGATCATACCAT |
| Suggested primer 2: | TGTGTTCTTCTGCCTGTTCG |