| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.041810 |
| Chromosome: | chromosome 2 |
| Location: | 2268933 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g090350 | (1 of 1) K16761 - centrosomal protein CEP44 (CEP44) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGTACTGAAGCAAGCAAACCGTGCAGCC |
| Internal bar code: | GACCCTCGTCCCCGGGGCTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 154 |
| LEAP-Seq percent confirming: | 99.7312 |
| LEAP-Seq n confirming: | 2226 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAACAGCTTGTACGTGCG |
| Suggested primer 2: | AATGCAATTTATTACGCCGC |