| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.041835 |
| Chromosome: | chromosome 14 |
| Location: | 2235508 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623000 | PGP7 | Phosphoglycolate phosphatase/2-deoxygucose-6-phosphate phosphatase; (1 of 1) PTHR18901//PTHR18901:SF31 - 2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2 // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAACGAACCCCCACTTACCTGCACAATC |
| Internal bar code: | ATGAAACGCGAGTTGACGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 466 |
| LEAP-Seq percent confirming: | 99.7245 |
| LEAP-Seq n confirming: | 6877 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCTACTCAACGACTCCTC |
| Suggested primer 2: | AAGCAGGCCAAGTAGGGAAT |