| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.041840 |
| Chromosome: | chromosome 13 |
| Location: | 552479 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g565301 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGACCATGACCTTGGGGCCCAGCAGGTC |
| Internal bar code: | GCCTCACGTCCTAGCGGCTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1022 |
| LEAP-Seq percent confirming: | 99.2424 |
| LEAP-Seq n confirming: | 917 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCGAGACAAACGTGAACA |
| Suggested primer 2: | TCCAGCACTATGCTCACCAG |