| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.041845 |
| Chromosome: | chromosome 12 |
| Location: | 5407239 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529950 | EIF4G1,EIF4G | (1 of 2) K03260 - translation initiation factor 4G (EIF4G); Eukaryotic translation initiation factor 4, gamma subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTTGGTGGTCGGGTACAGAGGTTTGTG |
| Internal bar code: | AATGTACATTTTTTGCCCTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1068 |
| LEAP-Seq percent confirming: | 89.1508 |
| LEAP-Seq n confirming: | 2572 |
| LEAP-Seq n nonconfirming: | 313 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGAAGGCAAGTTGGTAA |
| Suggested primer 2: | TAATGGTGGCCTCTACTGGG |