Insertion junction: LMJ.RY0402.041858_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.041858
Chromosome:chromosome 9
Location:382005
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g404900 (1 of 45) IPR029052 - Metallo-dependent phosphatase-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCTCGTCACCTCCACCCCGCCCGCAAG
Internal bar code:AAGTATCGATACTAAACCCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:54
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AAACCATTCCCGATAGTCCC
Suggested primer 2:GACAGCAAGTCGCATGTCAA