| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.041863 |
| Chromosome: | chromosome 10 |
| Location: | 106506 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418400 | (1 of 2) PF08590 - Domain of unknown function (DUF1771) (DUF1771) | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGTACAGGACAGGGTAAGGGCAGGTTGG |
| Internal bar code: | TCGTTCTTTGCGGATATTGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 293 |
| LEAP-Seq percent confirming: | 99.5457 |
| LEAP-Seq n confirming: | 3506 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCGCAGTCGCCTATAGTA |
| Suggested primer 2: | CCTGGCAACCTTTTGTGAAT |