Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.041903 |
Chromosome: | chromosome 1 |
Location: | 7511282 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g054100 | (1 of 21) IPR001611//IPR003591 - Leucine-rich repeat // Leucine-rich repeat, typical subtype | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAAGCTTGCGGCCGCCGCTCAGTGCAAAC |
Internal bar code: | GTAGGGAGGGAAGCGACCGGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 884 |
LEAP-Seq percent confirming: | 99.7181 |
LEAP-Seq n confirming: | 2830 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGCTTGGCAAGATTGATTA |
Suggested primer 2: | TGATACTGCGACTGCTACCG |