| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.041923 |
| Chromosome: | chromosome 8 |
| Location: | 3578489 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g375751 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACTTGCAAGTCCGCTCTACCTTGGCGGC |
| Internal bar code: | TTCGGAATTGCGGCCTTGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 429 |
| LEAP-Seq percent confirming: | 7.0783 |
| LEAP-Seq n confirming: | 885 |
| LEAP-Seq n nonconfirming: | 11618 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTGCAGAAGGACTGACG |
| Suggested primer 2: | ATGCGTAGATGACCCTGACC |