Insertion junction: LMJ.RY0402.041962_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.041962
Chromosome:chromosome 2
Location:3637470
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095145 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATAATCCCATTGAATCCGTGAATGTCCGC
Internal bar code:ACAGGTGGCTGCCCTGTGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:847
LEAP-Seq percent confirming:51.4644
LEAP-Seq n confirming:5781
LEAP-Seq n nonconfirming:5452
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GGTCGAAGAGTGCCAGGTAG
Suggested primer 2:GGGCCGCTATTCTACATTGA