Insertion junction: LMJ.RY0402.041990_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.041990
Chromosome:chromosome 12
Location:1805821
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g512050 (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTGACGAGCAGCCAGGACCCGGAGATGC
Internal bar code:TTTAACGTTTAGGTTCACTTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1103
LEAP-Seq percent confirming:97.1845
LEAP-Seq n confirming:2865
LEAP-Seq n nonconfirming:83
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTGGTTGTCCTTGTGCTT
Suggested primer 2:GTGGGAAGGGGATTGTTTTT