| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.042029 |
| Chromosome: | chromosome 13 |
| Location: | 2113762 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g577700 | (1 of 100) IPR001810 - F-box domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGACCTGGCACATGCCATCGCTACTGCT |
| Internal bar code: | GATGGGACAGTATTCCCATTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 418 |
| LEAP-Seq percent confirming: | 99.824 |
| LEAP-Seq n confirming: | 1702 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGACACTGTAGCCCCTGT |
| Suggested primer 2: | GGAGGTCTTGATGGACTTGC |