| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.042032 |
| Chromosome: | chromosome 6 |
| Location: | 591274 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g253200 | PKY1 | Protein tyrosine kinase; (1 of 1) PTHR23257:SF534 - SERINE/THREONINE-PROTEIN KINASE ROCO7-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTAGCATAAATGTCTGTAACAAGCCAAG |
| Internal bar code: | CCGGTGCCAGGCAGGGTAGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1086 |
| LEAP-Seq percent confirming: | 99.7094 |
| LEAP-Seq n confirming: | 16470 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATATGAGACGCTTTGCGCT |
| Suggested primer 2: | CAGTGAGTCCTAGCCAAGCC |