Insertion junction: LMJ.RY0402.042036_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.042036
Chromosome:chromosome 9
Location:481120
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g404250 SRR29, CEP9,CEP9 Cysteine endopeptidase; (1 of 1) PF00112//PF01476//PF07645 - Papain family cysteine protease (Peptidase_C1) // LysM domain (LysM) // Calcium-binding EGF domain (EGF_CA) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACATATGGATACTAAGACATTCTCACTGG
Internal bar code:ACAGCAACTGTACCTTTTGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:879
LEAP-Seq percent confirming:99.1142
LEAP-Seq n confirming:3021
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGGTGAGGCATCTCGTAT
Suggested primer 2:TCCAACGGAGTACTCGATCC