Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.042101 |
Chromosome: | chromosome_3 |
Location: | 5016348 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g180550 | NTD5,FPN2 | 5\'-nucleotidase | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGCCCACGAGTCAGCCGCGACGTGCCTCAA |
Internal bar code: | TGCTGTCTGGATTTAATCGTTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 139 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 416 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTACTACTGCATCTCGCCC |
Suggested primer 2: | CGCGGGGTGTACTTACTGAT |