Insertion junction: LMJ.RY0402.042101_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.042101
Chromosome:chromosome 7
Location:904529
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g318900 DNJ2 (1 of 1) PF00226//PF13637 - DnaJ domain (DnaJ) // Ankyrin repeats (many copies) (Ank_4); DnaJ-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCGCACACTCTGACCGGCTTCACGCTGT
Internal bar code:CGGATCCTAACAATCATGTCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:482
LEAP-Seq percent confirming:99.4444
LEAP-Seq n confirming:1969
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CATTGTCCTGCACGTATTGC
Suggested primer 2:CGCTTGTGAAGGAGTGGATT