| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.042136 |
| Chromosome: | chromosome 2 |
| Location: | 5959781 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112000 | AOT7 | Amino acid transporter; (1 of 1) K14997 - solute carrier family 38 (sodium-coupled neutral amino acid transporter), member 11 (SLC38A11) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGTCAATGTCCCGTTGCACGGCAAGGT |
| Internal bar code: | TGGGTGCCCGGGAGCCCTTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 951 |
| LEAP-Seq percent confirming: | 99.5374 |
| LEAP-Seq n confirming: | 12909 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCACGGAAATACGGAAC |
| Suggested primer 2: | TTTTGACGAACAGTTTTGCG |