| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.042146 |
| Chromosome: | chromosome 16 |
| Location: | 127822 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g695300 | (1 of 1) K12878 - THO complex subunit 1 (THOC1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACGCACACGTGCACCTGCAGGTGTTTG |
| Internal bar code: | GGTTCGCTTGATGGGCAGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 361 |
| LEAP-Seq percent confirming: | 96.7199 |
| LEAP-Seq n confirming: | 5809 |
| LEAP-Seq n nonconfirming: | 197 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGTGTGTGTGTGTCCC |
| Suggested primer 2: | CCCACTAATGTCACAATGCG |