Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.042157 |
Chromosome: | chromosome_9 |
Location: | 2144219 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g393450 | FAP233 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGTGGTGCGGAAGCCCTGCACACACACAGT |
Internal bar code: | GAGGCCTGGACTGACCGCAAAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 238 |
LEAP-Seq percent confirming: | 99.7262 |
LEAP-Seq n confirming: | 1821 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGACACCCCCTTTTCTCTTG |
Suggested primer 2: | CCTAGTCGTGTTGCCCAAAT |