| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.042167 |
| Chromosome: | chromosome 10 |
| Location: | 2005844 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g432550 | KCN2 | Six-transmembrane domain potassium channel alpha subunit; (1 of 1) PTHR11537:SF133 - PROTEIN EXP-2, ISOFORM C | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCTCCGGTAACCACATGCAGACACGCGT |
| Internal bar code: | TCGACGTTGGGATCCGGAATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 99.1845 |
| LEAP-Seq n confirming: | 1946 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCCCTTCACTCCTGAAT |
| Suggested primer 2: | CGGATGAATGGCCTAGAAAA |