| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.042167 |
| Chromosome: | chromosome 10 |
| Location: | 2005852 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g432550 | KCN2 | Six-transmembrane domain potassium channel alpha subunit; (1 of 1) PTHR11537:SF133 - PROTEIN EXP-2, ISOFORM C | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGGGGCCATTGGCCGTGAGGGGCTGAG |
| Internal bar code: | CTCGTTGCCTGCAGGAAGACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1054 |
| LEAP-Seq percent confirming: | 99.2621 |
| LEAP-Seq n confirming: | 5112 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCCCTTCACTCCTGAAT |
| Suggested primer 2: | CGGATGAATGGCCTAGAAAA |