| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.042170 |
| Chromosome: | chromosome 6 |
| Location: | 1472783 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259500 | CYG2 | Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTCCTCTGCTGCGGTCACCGGCTCAAAA |
| Internal bar code: | CAAGCAGGATTCGTGATGTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 174 |
| LEAP-Seq percent confirming: | 72.8961 |
| LEAP-Seq n confirming: | 2590 |
| LEAP-Seq n nonconfirming: | 963 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGATCAGCCACCCCAG |
| Suggested primer 2: | ATGCCTTGTCCTGGATTCTG |