| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.042181 |
| Chromosome: | chromosome 4 |
| Location: | 3757170 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g229472 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACATCTTGTTGACCGACCCCCATGTCCCG |
| Internal bar code: | CGATATACGTGGCGGAAAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 948 |
| LEAP-Seq percent confirming: | 99.7122 |
| LEAP-Seq n confirming: | 6236 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAACGCACAGGTTTATTT |
| Suggested primer 2: | GCAGTCCTTGAGAGTCTGGG |