| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.042192 |
| Chromosome: | chromosome 3 |
| Location: | 2149506 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157250 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGCTGTGGGGCAAATGTAACAGCGCCA |
| Internal bar code: | GATGGAGGGGGCGTTTATAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 85 |
| LEAP-Seq percent confirming: | 89.8396 |
| LEAP-Seq n confirming: | 168 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTCCAGGTGTTGGTCAG |
| Suggested primer 2: | GTGTAGCTCGGGGTGGTTTA |