| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.042200 |
| Chromosome: | chromosome 7 |
| Location: | 3320503 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334700 | ATG101 | (1 of 1) PF07855 - Protein of unknown function (DUF1649) (DUF1649); Autophagy-related protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCTCGAGGCTGAGCAAGCTGTGTTGCC |
| Internal bar code: | GGAACCTTGGGTCGGCCCACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 654 |
| LEAP-Seq percent confirming: | 99.4118 |
| LEAP-Seq n confirming: | 169 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCTGCTGGCTGTTGTTGA |
| Suggested primer 2: | AAGGCCGAAGTACAGAAGCA |