Insertion junction: LMJ.RY0402.042205_4

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.042205
Chromosome:chromosome 11
Location:3113424
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g479050 FAP229,FKB15-1,FKB15A,FKB1 Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 1) PTHR10516//PTHR10516:SF293 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAAAGACATCCCGGTTCAGTGTTTGGACT
Internal bar code:AGTTCACCCGATAAGGATTATC

Confirmation - LEAP-Seq

LEAP-Seq distance:148
LEAP-Seq percent confirming:98.5366
LEAP-Seq n confirming:202
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTCCTTTACACGCCCTTG
Suggested primer 2:TGCCTTGTCATTTCAAGCAG