| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.042217 |
| Chromosome: | chromosome 3 |
| Location: | 8625804 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203345 | (1 of 21) IPR001357 - BRCT domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATGTTGTCCGGTGGTCGTACGCGAACT |
| Internal bar code: | GGCCCGCTGATCCACGGTGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 530 |
| LEAP-Seq percent confirming: | 99.6603 |
| LEAP-Seq n confirming: | 1467 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTGAGATGCTTTGGGAG |
| Suggested primer 2: | ATGCGACCTATCTCACCTGG |