Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.042278 |
Chromosome: | chromosome 6 |
Location: | 3933553 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g278187 | MOT4 | (1 of 1) K15716 - E3 ubiquitin-protein ligase ZSWIM2 [EC:6.3.2.19] (ZSWIM2); Predicted protein | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCATCCTACGTACCCAGGCCACCCCGC |
Internal bar code: | AGTGGTCTGACTACGTTAGATT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 662 |
LEAP-Seq percent confirming: | 99.4587 |
LEAP-Seq n confirming: | 735 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAGGCCCTACACACTGGAC |
Suggested primer 2: | CAGCTAATTCCCAAACCCAA |