| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.042287 |
| Chromosome: | chromosome 12 |
| Location: | 9129912 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542569 | (1 of 4) PF00497 - Bacterial extracellular solute-binding proteins, family 3 (SBP_bac_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTTAAAGGGGCACAGATATTTGACTGGG |
| Internal bar code: | GAATCATCCCCCTTTGCGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 820 |
| LEAP-Seq percent confirming: | 81.0502 |
| LEAP-Seq n confirming: | 1420 |
| LEAP-Seq n nonconfirming: | 332 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTGGACAACCCAGACTTA |
| Suggested primer 2: | TCGGCACACACTTAGACCAC |